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       ÄÚ¸®³×¹ÚÅ׸®¿ò ±â¹Ý ÇÕ¼º»ý¹°ÇÐÀû ¿¬±¸ µ¿Çâ
       kimsg7596@kaist.ac.kr        
       Á¤±âÁØ ±³¼ö        2014.11.21 15:19        18577
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ÄÚ¸®³×¹ÚÅ׸®¿ò ±â¹Ý ÇÕ¼º»ý¹°ÇÐÀû ¿¬±¸ µ¿Çâ

 

 

 KAIST Á¤±âÁØ ±³¼ö,  ÀÓ¼º¼ø ¹Ú»ç°úÁ¤

 

1. °³¿ä
ºÐÀÚ»ý¹°ÇÐÀÇ ¹ßÀü°ú ´õºÒ¾î À¯Àü°øÇÐ ±â¼úµéÀÇ ¹ßÀüÀ» ÅëÇØ¼­ ÇÕ¼º»ý¹°ÇÐ »ý¹°ÇÐÀû ½Ã½ºÅÛ ¿£Áö´Ï¾î¸µ ±â¼úÀº »ý¸í°øÇÐ ½Ã´ë¿¡ »õ·Î¿î »ê¾÷ ºÐ¾ß¸¦ âÃâÇÏ¸ç »ý¸í°úÇÐ »ê¾÷ÀÇ ¸ð½ÀÀ» ºü¸£°Ô º¯È­½Ã۰í ÀÖ´Ù. ƯÈ÷ ÇÕ¼º»ý¹°ÇÐÀº ¹ÙÀÌ¿ÀÀÇ¾à »Ó¸¸ ¾Æ´Ï¶ó White BT, Green BTÀÇ º»°ÝÀû ¼ºÀåÀ» °¡´ÉÄÉ ¸¸µé °ÍÀ¸·Î ±â´ëµÇ°í ÀÖ´Ù. ±× µ¿¾ÈÀÇ ÇÕ¼º»ý¹°ÇÐ ±â¹ÝÀÇ ±â¼úµéÀº ´ëÀå±Õ, È¿¸ð¿Í °°ÀÌ ´Ù¾çÇÑ genetic toolÀÌ È®¸³µÇ¾î ÀÖ´Â ¹Ì»ý¹°¿¡ ±â¹ÝÇØ¼­ °³¹ß ¹× ÀÀ¿ëÀÌ µÇ¾î¿À°í ÀÖ¾ú´Âµ¥, Á¡Â÷ Bacillus subtilis, Pichia pastoris, Streptomyces avermitilis, cyanobacteria±îÁö ±× ´ë»ó ¹Ì»ý¹°ÀÇ ¹üÀ§°¡ ³Ð¾îÁö°í ÀÖ´Ù.
Corynebacterium glutamicum (ÄÚ¸®³×¹ÚÅ׸®¿ò ±Û·çŸ¹ÌÄñ)Àº Æ÷ÀÚ¸¦ Çü¼ºÇÏÁö ¾Ê´Â ±×¶÷ ¾ç¼º±ÕÀ̸ç, º´¿ø¼ºÀÌ ¾ø´Â GRAS (Generally recognized as safe) ±ÕÁÖ·Î½á ´Ù¾çÇÑ L-¾Æ¹Ì³ë»ê, ÇÙ»ê µîÀ» »ý»êÇÏ´Â µ¥¿¡ »ê¾÷ÀûÀ¸·Î ³Î¸® »ç¿ëµÇ°í ÀÖ´Â À¯¿ëÇÑ »ê¾÷ ±ÕÁÖÀÌ´Ù.

¶ÇÇÑ ÄÚ¸®³×¹ÚÅ׸®¿òÀº ¾Æ¹Ì³ë»ê »ý»ê»Ó¸¸ ¾Æ´Ï¶ó ±×¶÷ ¾ç¼º±ÕÀÇ Æ¯¼ºÀ¸·Î ÀÎÇÑ ¿ëÀÌÇÑ ´Ü¹éÁú ºÐºñ »ý»ê¿¡¼­µµ Å« ÀåÁ¡À» °¡Áö°í ÀÖ´Ù. ±×¸®°í ÇöÀçÀÇ ¾Æ¹Ì³ë»êÀ̳ª ´Ü¹éÁú »ý»êÀ» ³Ñ¾î ÄÚ¸®³×¹ÚÅ׸®¿òÀ» ÅëÇØ ´Ù¾çÇÑ ¹ÙÀÌ¿À È­ÇÕ¹°À» »ý»êÇϰíÀÚ ÇÏ´Â ¿¬±¸°¡ Ȱ¹ßÈ÷ ÁøÇàµÇ°í ÀÖ´Ù. º» º¸°í¼­¿¡¼­´Â ÀÌ·¸°Ô À¯¿ëÇÑ »ê¾÷±ÕÁÖ Corynebacterium glutamicum (ÄÚ¸®³×¹ÚÅ׸®¿ò ±Û·çŸ¹ÌÄñ) ±â¹ÝÀÇ ÇÕ»ý»ý¹°ÇÐÀû ¿¬±¸ µ¿Çâ¿¡ ´ëÇØ¼­ °íÂûÇØ º¸°íÀÚ ÇÑ´Ù.


2. ÄÚ¸®³×¹ÚÅ׸®¿ò tunable gene expression system ¿¬±¸ µ¿Çâ
ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ ´Ù¾çÇÑ ¸ñÇ¥ È­ÇÕ¹°À» °íÈ¿À²·Î »ý»êÇÏ·Á¸é ¸ñÇ¥ È­ÇÕ¹°·ÎÀÇ ´ë»ç È帧À» ÃÖÀûÀ¸·Î Á¶ÀýÇØÁÖ´Â °Í»Ó¸¸ ¾Æ´Ï¶ó Çʿ信 µû¶ó¼­´Â »õ·Î¿î ÇÕ¼º ´ë»ç °æ·Î¸¦ ±¸ÃàÇØ¾ß Çϴµ¥ À̸¦ À§Çؼ­´Â ´Ù¾çÇÑ À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¤±³ÇÏ°Ô Á¶ÀýÇÒ ¼ö ÀÖ´Â ±â¼úÀÌ ÇÊ¿äÇÏ´Ù. ±âÁ¸ ÄÚ¸®³×¹ÚÅ׸®¿òÀÇ À¯ÀüÀÚ ¹ßÇö Á¶ÀýÀ» À§Çؼ­´Â º¸Åë °­ÇÑ ¹ßÇö Á¤µµ°¡ ¾Ë·ÁÁ® ÀÖ´Â ¸î¸î °³ÀÇ ÄÚ¸®³×¹ÚÅ׸®¿ò À¯·¡ÀÇ ÇÁ·Î¸ðÅÍ(PsodA, PgapA, Peftu, PcspB)³ª ´ëÀå±Õ À¯·¡ÀÇ ÇÁ·Î¸ðÅÍ(Plac, Ptrc, Ptac) µîÀÌ ÀÌ¿ëµÇ¾î ¿Ô´Ù. ÇÏÁö¸¸ °¢°¢ÀÇ ÇÁ·Î¸ðÅÍÀÇ »ó´ëÀûÀÎ ¼¼±â´Â Á÷Á¢ÀûÀ¸·Î ºñ±³µÈ ¹Ù°¡ ¾øÀ¸¸ç, °­ÇÏ´Ù°í ¾Ë·ÁÁ® ÀÖ´Â ÇÁ·Î¸ðÅ͸¦ »ç¿ëÇÏ´õ¶óµµ 5¡¯UTR (untranslated region)°ú ¸ñÇ¥ À¯ÀüÀÚ ¼­¿­°úÀÇ Á¶ÇÕ µîÀÇ È¿°ú¿¡ ÀÇÇØ ´Þ¶óÁú ¼ö ÀÖ´Â ¹ßÇö È¿À² ¶§¹®¿¡ ´Ù¾çÇÑ ¼¼±âÀÇ À¯ÀüÀÚ ¹ßÇöÀ» ¸ñÇ¥·Î ÇÒ ¶§, ¸ñÇ¥ À¯ÀüÀÚ¿¡ ´ëÇØ¼­ ÃÖÀûÀÇ ¼¼±â¸¦ º¸ÀÌ´Â °¢°¢ÀÇ ÇÁ·Î¸ðÅ͸¦ ¼±º°ÇÏ´Â °ÍÀÌ ¸Å¿ì ¾î·Á¿î ½ÇÁ¤À̾ú´Ù.

 

±×¸² 1. ÄÚ¸®³×¹ÚÅ׸®¿ò ±â¹Ý ÇÕ¼º ÇÁ·Î¸ðÅÍ ¶óÀ̺귯¸®ÀÇ ±¸Ãà ¹× FACS¸¦ ÅëÇÑ

´Ù¾çÇÑ ¼¼±âÀÇ ÇÁ·Î¸ðÅÍ ¹ß±¼

 

µû¶ó¼­ ÀÌ·¯ÇÑ ¹®Á¦Á¡À» ÇØ°áÇϰíÀÚ 2013³â º» ¿¬±¸ÁøÀº ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ GFP¸¦ º¸°í ´Ü¹éÁú·Î Ȱ¿ëÇÑ ÇÕ¼º ÇÁ·Î¸ðÅÍ ¶óÀ̺귯¸®¸¦ ±¸ÃàÇÏ¿´°í, À̸¦ FACS (Fluorescence-Activated Cell Sorting) screeningÀ» ÅëÇØ ´Ù¾çÇÑ ¼¼±â¸¦ º¸ÀÌ´Â constitutive ÇÕ¼º ÇÁ·Î¸ðÅ͵éÀ» ¹ß±¼ÇÒ ¼ö ÀÖ¾ú´Ù(±×¸² 1). ¿©±â¼­ ¹ß±¼µÈ °ÅÀÇ ´ëºÎºÐÀÇ ÇÕ¼º ÇÁ·Î¸ðÅ͵éÀº 5¡¯UTRÀ» Æ÷ÇÔÇÏÁö ¾ÊÀ¸¸ç ¸ñÇ¥ À¯ÀüÀÚÀÇ ATG °³½ÃÄÚµ·ºÎÅÍ Àü»ç¸¦ ½ÃÀÛÇÏ´Â leaderless transcriptionÀ» º¸ÀÌ´Â °ÍÀ¸·Î È®ÀÎ µÇ¾ú´Âµ¥, leaderless transcription systemÀº °¢°¢ÀÇ ¸ñÇ¥ À¯ÀüÀÚÀÇ ¹ßÇö¿¡ ´ëÇØ¼­ 5¡¯UTRÀÇ È¿°ú¸¦ ¹èÁ¦Çϰí ÇÁ·Î¸ðÅÍÀÇ ¼¼±â¸¸À» °¡Áö°í ¿¹Ãø °¡´ÉÇÑ ¹ßÇö ½Ã½ºÅÛÀ» ±¸ÃàÇÒ ¼ö Àֱ⿡ ±âÁ¸ÀÇ »ç¿ëµÇ¾î¿À´ø À¯ÀüÀÚ ¹ßÇö ½Ã½ºÅÛ¿¡ ºñÇØ¼­ ¸Å¿ì Å« ÀåÁ¡À» °¡Áø´Ù°í ÇÒ ¼ö ÀÖ°Ú´Ù. ¶ÇÇÑ ÃÖ±Ù¿¡´Â Rytter et al., 2014.¿¡¼­ ÄÚ¸®³×¹ÚÅ׸®¿ò ÇÁ·Î¸ðÅ͵鿡¼­ È®ÀÎµÈ consensus sequence¸¦ ÀÌ¿ëÇÏ¿© ´Ù¾çÇÑ Á¾·ùÀÇ ÇÕ¼º ÇÁ·Î¸ðÅÍ ¶óÀ̺귯¸®¸¦ ±¸ÃàÇÏ¿© ´Ù¾çÇÑ ¼¼±âÀÇ ÇÕ¼º ÇÁ·Î¸ðÅ͸¦ ±¸ÃàÇÏ¿´À» »Ó¸¸ ¾Æ´Ï¶ó, ´ëÀå±Õ lac ÇÁ·Î¸ðÅÍ¿¡ ±â¹ÝÇÑ ÇÁ·Î¸ðÅÍ ¶óÀ̺귯¸®¸¦ ÅëÇØ ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ ´Ù¾çÇÑ ¼¼±â¸¦ º¸ÀÌ´Â IPTG-inducible ÇÁ·Î¸ðÅÍµé ¶ÇÇÑ °³¹ßÇßÀ½À» º¸°íÇÏ¿´´Ù.
Promoter ¿µ¿ª¿¡¼­»Ó¸¸ ¾Æ´Ï¶ó ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ Á¡Á¡ ´õ ¸¹Àº DNA partµéÀÌ °³¹ßµÇ¸é¼­ À̸¦ Á¶ÇÕÇØ¼­ ½ÃÇèÇÏ°í »ç¿ëÇÒ ¼ö ÀÖµµ·Ï ÇØÁÙ ÇÕ¼º»ý¹°ÇÐ ±â¹ÝÀÇ plasmid platformÀÌ ÇÊ¿äÇÏ°Ô µÇ¾ú´Âµ¥ ÀÌ´Â 2012³â Ravasi et al., 2012.ÀÇ pTGR platform°ú 2014³â Kang et al., 2014.ÀÇ CoryneBrick platform¿¡ ÀÇÇØ¼­ ±¸ÃàµÇ¾ú´Ù. µÎ platform ¸ðµÎ ¸ñÇ¥ À¯ÀüÀÚÀÇ ¹ßÇöÀ» À§ÇÑ ÇÁ·Î¸ðÅÍ, RBS, Terminator, Selection marker, E. coli origin, C. glutamicum origin µîÀ» Ư¼öÇÑ Á¦ÇÑ È¿¼Ò¸¦ ÅëÇØ¼­ Á¶ÇÕÇÏ°í ½ÃÇèÇÒ ¼ö ÀÖ´Â ½Ã½ºÅÛÀÌ´Ù. ƯÈ÷ CoryneBrick platformÀº ¸ñÇ¥ À¯ÀüÀÚµéÀ» ¿øÇÏ´Â ´ë·Î Á¶ÇÕÇϰí Á¶¸³Çϱ⠽¬¿î BglBrick Ŭ·Î´× ¹æ¹ýÀ» µµÀÔÇÏ¿© ´õ °£ÆíÇÑ ÇÕ¼º»ý¹°ÇÐ ±â¹Ý plasmid platformÀ̶ó ÇÒ ¼ö ÀÖ°Ú´Ù.

 

3. ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ÀÇ metabolite biosensor ¿¬±¸ µ¿Çâ
ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ ¸ñÇ¥ metabolite¸¦ °íÈ¿À²·Î ´ë·®»ý»ê Çϱâ À§ÇØ ¸¹Àº ´ë»ç°øÇÐÀû Á¢±ÙÀÌ ÀÌ·ç¾îÁ³´Ù. ±×¸®°í ´ë»ç°øÇÐÀÇ ¿©·¯°¡Áö ÇѰèÁ¡À» ±Øº¹Çϱâ À§ÇØ ÇÕ¼º»ý¹°ÇÐ °³³äÀ» µµÀÔÇÏ¿© ´õ ºü¸£°í Á¤È®ÇÏ°Ô ¸ñÇ¥ È­ÇÕ¹° »ý»ê ½Ã½ºÅÛÀ» ±¸ÃàÇϰíÀÚ ÇÏ´Â ¿¬±¸°¡ Ȱ¹ßÈ÷ ÁøÇàµÇ°í Àִµ¥ ´ëÇ¥ÀûÀ¸·Î ¼¼Æ÷ ³» Transcription factor¸¦ ÅëÇØ ¼¼Æ÷ ³»ÀÇ ´ë»ç»ê¹°ÀÇ ¾çÀ» °¨ÁöÇÏ´Â biosensor ½Ã½ºÅÛÀÌ ÀÖ´Ù. Biosensor ½Ã½ºÅÛÀº ¼¼Æ÷ ³»¿¡ Á¸ÀçÇÏ´Â ´ë»ç»ê¹°ÀÇ ¾çÀ» °¨ÁöÇÏ¿© ÇÊ¿äÇÑ À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö Àִµ¥, À̸¦ ÀÌ¿ëÇÏ¿© ´ë»ç»ê¹°ÀÇ ¾çÀ» Çü±¤ ´Ü¹éÁúÀÇ ¹ßÇöÀ¸·Î ¿¬°áÇÏ¿© FACS¸¦ ÅëÇÏ¿© ºÐ¼®Çϰí, ±×¸¦ ÅëÇØ ÃÖÀû À¯ÀüÀÚ ¹ßÇö ½Ã½ºÅÛ ±¸Ãà, È¿¼Ò engineering ¶Ç´Â genome engineeringÀ» high-throughputÀ¸·Î ¼öÇàÇÏ´Â ¿¬±¸°¡ ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­µµ º¸°íµÇ¾ú´Ù.

 

 

±×¸² 2. ÄÚ¸®³×¹ÚÅ׸®¿òÀÇ ¼¼Æ÷ ³» ¶óÀ̽Š³óµµ¿¡ µû¶ó¼­ EYFP¸¦ ¹ßÇö½ÃŰ´Â

¶óÀ̽м¾¼­ ½Ã½ºÅÛ

 

µ¶ÀÏÀÇ Lothar eggeling groupÀÌ ÄÚ¸®³×¹ÚÅ׸®¿òÀÇ metabolite sensor¸¦ ÅëÇØ ¸¹Àº ¿¬±¸¸¦ ¼öÇàÇϰí Àִµ¥ ´ëÇ¥ÀûÀ¸·Î´Â ¼¼Æ÷ ³» ¶óÀ̽г󵵰¡ ³ô¾ÆÁú¼ö·Ï ¶óÀ̽ÅÀ» ¼¼Æ÷ ¹ÛÀ¸·Î ¹èÃâÇϱâ À§ÇÑ ¶óÀ̽Šexporter LysEÀÇ ¹ßÇö¿¡ °ü·ÃµÈ LysG transcriptional regulator¿Í lysE À¯ÀüÀÚÀÇ ÇÁ·Î¸ðÅ͸¦ ÀÌ¿ëÇÏ¿© ¶óÀ̽Š³óµµ¿¡ µû¶ó EYFP¸¦ »ý»êÇÏ´Â ¶óÀ̽м¾¼­¸¦ ±¸ÃàÇÑ ¿¹°¡ ÀÖ´Ù. ±×¸®°í ÀÌ·¯ÇÑ ¾Æ¹Ì³ë»ê ¼¾¼­¸¦ ÀÌ¿ëÇÏ¿© ÄÚ¸®³×¹ÚÅ׸®¿ò À¯Àüü µ¹¿¬º¯ÀÌ¿¡¼­ ¶óÀ̽Š´ë·® »ý»ê¿¡ µµ¿òÀÌ µÇ´Â »õ·Î¿î mutation µîÀ» ½Äº°ÇÏ´Â ¿¬±¸, ¾ËÁö´Ñ¿¡ feedback inhibitionÀ» ´ú ¹Þ´Â ArgB È¿¼Ò¸¦ engineering ¿¬±¸ µîÀ» FACS¸¦ ÅëÇÏ¿© High-throughputÀ¸·Î ¼öÇàÇÏ¿© º¸°íÇÏ¿´´Ù (±×¸² 2). ÀÌ´Â ÇÕ¼º»ý¹°Çп¡ ±â¹ÝÇÑ À¯ÀüÀÚ ¹ßÇö ½Ã½ºÅÛ ±¸ÃàÀ» ÅëÇØ¼­ ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ ´ë»ç»ê¹°À» Á¤È®ÇÏ°Ô °¨ÁöÇϰí À̸¦ ÅëÇØ ´Ù¾çÇÑ engineeringÀ» ½Å¼ÓÇÏ°Ô ÇÒ ¼ö ÀÖ´Ù´Â °ÍÀ» º¸¿©ÁÖ´Â °á°ú¶ó ÇÒ ¼ö ÀÖ°Ú´Ù.

 

4. ÄÚ¸®³×¹ÚÅ׸®¿òÀÇ ÇÕ¼º»ý¹°ÇÐ ±â¹Ý genome engineering ¿¬±¸ µ¿Çâ

 

±×¸² 3. ProphageÀÇ RecT recombinase¸¦ ÅëÇÑ genome engineering°ú

¶óÀ̽йÙÀÌ¿À ¼¾¼­ ½Ã½ºÅÛÀ» ÀÌ¿ëÇÑ ÄÚ¸®³×¹ÚÅ׸®¿ò ±ÕÁÖ °³¹ß

 

ÄÚ¸®³×¹ÚÅ׸®¿òÀÇ genome engineeringÀº ´ëü·Î ½Ã°£ÀÌ ¸¹ÀÌ µé°í ¼ÕÀÌ ¸¹ÀÌ °¡´Â ÀüÅëÀûÀÎ ¹æ¹ýÀ¸·Î homologous recombination¿¡ ÀÇÇÑ gene knock-out ȤÀº integrationÀ» ÅëÇØ¼­ ÀÌ·ç¾îÁ®¿Ô´Ù. ÇÏÁö¸¸ ÃÖ±Ù Binder et al., 2013¿¡ º¸°íµÈ ¹Ù¿¡ µû¸£¸é single-strand DNA¿Í prophage recombinase RecT¸¦ ÀÌ¿ëÇÏ¿© genomeÀÇ Æ¯Á¤ÇÑ À§Ä¡¿¡ Á¤È®ÇÏ°Ô mutationÀ» ÀÏÀ¸Å°°í, ¸ñÇ¥·Î ÇÏ´Â ´ë»ç»ê¹°ÀÇ ¾çÀ» °¨ÁöÇÏ¿© ±× ¾ç¿¡ µû¶ó Çü±¤ ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â ¹ÙÀÌ¿À¼¾¼­¸¦ ÇÔ²² ÀÌ¿ëÇÏ¿© À¯ÀÍÇÑ mutationÀ» °¡Áö´Â µ¹¿¬º¯À̸¦ FACS¸¦ ÅëÇÏ¿© ¹ß±¼Çس»´Â RecFACS ±â¼úÀÌ °³¹ßµÇ¾ú°í, À̸¦ ÅëÇØ ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­µµ ´ÙÁßÀÇ genome engineeringÀÌ °¡´ÉÇÏ°Ô µÇ¾ú´Ù. ¶ÇÇÑ zinc-finger nucleases, transcription activator-like effector nucleases (TALENs) ±×¸®°í CRISPR-Cas nuclease system°ú °°Àº genome engineering tool ¶ÇÇÑ ÄÚ¸®³×¹ÚÅ׸®¿òÀÇ ´õ È¿°úÀûÀÎ ±ÕÁÖ °³¹ß¿¡ ÀÀ¿ëµÉ ¼ö ÀÖÀ» °ÍÀ¸·Î º¸ÀδÙ.

 

5. °íÂû
ÀÌ·¸µí ´Ù¾çÇÑ ÇÕ¼º»ý¹°ÇÐ ±â¹Ý genetic toolÀÌ ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­µµ ±¸ÃàÀÌ µÇ°í ÀÖÀ¸¸ç, À̸¦ ÅëÇØ¼­ ´õ¿í ´õ ½±°í ºü¸£°í È¿°úÀûÀÎ ÄÚ¸®³×¹ÚÅ׸®¿òÀÇ engineeringÀÌ ¿ëÀÌÇØÁö°í ÀÖ´Ù. ÇÏÁö¸¸ ¿©ÀüÈ÷ ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ Ȱ¿ë °¡´ÉÇÑ engineering toolÀº ´ëÀå±Õ ¹× È¿¸ð¿¡ ºñÇØ¼­ ¸¹ÀÌ ºÎÁ·ÇÑ ½ÇÁ¤ÀÌ¸ç ¸¹Àº ¿¬±¸°¡ ´õ ÇÊ¿äÇÏ´Ù. ´ëÀå±Õ ¹× È¿¸ð¿¡ ºñÇØ¼­ »ê¾÷¿ë ±ÕÁַμ­ Å« ÀåÁ¡À» Áö´Ñ ÄÚ¸®³×¹ÚÅ׸®¿ò¿¡¼­ ´õ ´Ù¾çÇÑ ÇÕ¼º»ý¹°ÇÐÀûÀÎ genetic engineering toolÀÌ °³¹ßµÈ´Ù¸é White BT ºÐ¾ß¿¡ ¹ÌÄ¥ ¼ö ÀÖ´Â ÆÄ±ÞÈ¿°ú´Â ¸Å¿ì Ŭ °ÍÀ¸·Î ¿¹»óÇÑ´Ù.

 

Âü°í¹®Çå
1. ±èžï, ±è¼º¿ì. ÇÕ¼º»ý¹°ÇÐ (Synthetic biology) ¿¬±¸°³¹ß ¹× »ê¾÷È­ µ¿Çâ –

   ¹ÙÀÌ¿À»ê¾÷ ¹ü¿ë±â¼úÇ÷§Æû. »ý¸í°øÇÐÁ¤Ã¥¿¬±¸¼¾ÅÍ.
2. Woo HM, Park JB. 2014. Recent progress in development of synthetic biology platforms

    and metabolic engineering of Corynebacterium glutamicum. J Biotechnol 180:43-51.
3. Becker J, Wittmann C. 2011. Bio-based production of chemicals, materials and fuels –

   Corynebacterium glutamicum as versatile cell factory. Curr Opin Biotechnol 23:1-10.
4. Yim SS, An SJ, Kang M, Lee J, Jeong KJ. 2013. Isolation of fully synthetic promoters for

    high-level gene expression in Corynebacterium glutamicum. Biotechnol Bioeng

    110:2959-2971.
5. Rytter JV, Helmark S, Chen J, Lezyk MJ, Solem C, Jensen PR. 2014. Synthetic promoter

    libraries for Corynebacterium glutamicum. Appl Microbiol Biotechnol 98:2617-2623.
6. Ravasi P, Peiru S, Gramajo H, Menzella HG. 2012. Design and testing of a synthetic

    biology framework for genetic engineering of Corynebacterium glutamicum. Microbial Cell

    Fact 11:147.
7. Kang MK, Lee J, Um Y, Lee TS, Bott M, Park SJ, Woo HM. 2014. Synthetic biology

   platform of CoryneBrick vectors for gene expression in Corynebacterium glutamicum and

   its application to xylose utilization. Appl Microbiol Biotechnol 98:5991-6002.
8. Zhang F, Carothers JM, Keasling JD. 2012. Design of a dynamic sensor-regulator system

    for production of chemicals and fuels derived from fatty acids.

    Nat Biotechnol 30:354-359.
9. Binder S, Schendzielorz G, Stäbler N, Krumbach K, Hoffmann K, Bott M, Eggeling L. 2012.

    A high-throughput approach to identify genomic variats of bacterial metabolite producers

    at the single-cell level. Genome Biol 13:R40
10. Schendzielorz G, Dippong M, Grünberger A, Kohlheyer D, Yoshida A, Binder S,

     Nishiyama C, Nishiyama M, Bott M, Eggeling L. 2014. Taking control over control: use of

     product sensing in single cells to remove flux control at key enzymes in biosynthesis

     pathways. ACS Synth Biol 3:21-29.
11. Binder S, Siedler S, Marienhagen J, Bott M, Eggeling L. 2013. Recombineering in

     Corynebacterium glutamicum combined with optical nanosensors: a general strategy for

     fast producer strain generation. Nuc Acids Res 41:6360-6369.




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