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       Evolutionary adaptation ¹× reverse engineering ±â¹ÝÀÇ ÃÖ±Ù ¿¬±¸µ¿Çâ
       kimsg7596@kaist.ac.kr        
       À̼º±¹ ±³¼ö        2015.01.27 14:49        13278
´Ù¿î·Îµå : Evolutionary adaptation ¹× reverse engineering ±â¹ÝÀÇ ÃÖ±Ù ¿¬±¸µ¿Çâ_À̼º±¹ ±³¼ö.pdf(359 Kb)
 

Evolutionary adaptation ¹× reverse engineering ±â¹ÝÀÇ ÃÖ±Ù ¿¬±¸µ¿Çâ

 

¿ï»ê°úÇбâ¼ú´ëÇб³ À̼º±¹ ±³¼ö

 

1. ¿¬±¸¹è°æ

¿À´Ã³¯ ÇÕ¼º»ý¹°ÇÐ (Synthetic  biology)Àº »ý¹°°øÇÐ (Biotechnology) ±â¹ÝÀÇ ´Ù¾çÇÑ ¹ÙÀÌ¿ÀÈ­ÇÐ »ê¾÷ ºÐ¾ß¿¡ »õ·Î¿î Æз¯´ÙÀÓÀ» Á¦½ÃÇϸ鼭 ³ª³¯ÀÌ ºñ¾àÀûÀ¸·Î º¯È­ ¹ßÀüÇØ°¡°í ÀÖ´Ù. À̸¦ ¹Ý¿µÇϵí ÇÕ¼º»ý¹°ÇÐÀº ÃÖ±Ù È­Çй°Áú, ÀÇ¾à µî »ê¾÷Àû »ý»ê¿¡ ÀÖ¾î ÇÙ½ÉÀûÀÎ ±â¼ú·ÎÀÇ ÀáÀ缺À» ÀÎÁ¤¹Þ°í ÀÖ´Ù. ´õ¿íÀÌ ÇöÀç ´Ù¾çÇÑ genomics, transcriptomics, metabolomics, proteomics µî ¿À¹Í½º ±â¹ÝÀÇ ºÐ¼®±â¼ú°ú À¯ÀüÀÚ ÇÕ¼º±â¼úµéÀÇ ¹ßÀüÀ¸·Î ÇÕ¼º»ý¹°ÇÐÀÇ ¼ºÀå¼Óµµ´Â °¡¼ÓÈ­µÇ¾î °¡°í ÀÖ´Â ½ÇÁ¤ÀÌ´Ù. ÀÌ·Î ÀÎÇØ ¿¹Àü¿¡´Â ÇÒ ¼ö ¾ø¾úÁö¸¸ Áö±ÝÀº °¡´ÉÇÑ ¡®evolutionary adaptation¡¯°ú ¡®reverse engineering¡¯°ú °°Àº ±â¼úµéÀÌ °¢±¤À» ¹Þ°í ÀÖÀ¸¸ç ¹Ì»ý¹°ÀÇ ¿øÇϴ ǥÇöÇüÀ» ¼³°èÇϴµ¥ ÀÖ¾î Áß¿äÇÑ ±â¼ú·Î Àνĵǰí ÀÖ´Ù. ÀÚ¿¬°ú À¯»çÇÑ evolution ȯ°æÀ» ÀΰøÀûÀ¸·Î ÀçÇöÇÏ¿© ¹Ì»ý¹°ÀÇ Æ¯Á¤ Ç¥ÇöÇüÀ» ȹµæÇϱâ À§ÇÑ evolutionary engineeringÀº »ýüºÐÀÚ(biomolecule), ´ë»çȸ·Î (metabolic pathway), ³ª¾Æ°¡ »ý¹°Æ¯¼ºÀ» ÆľÇÇϴµ¥ ÀÖ¾î À¯¿ëÇÏ´Ù. ƯÈ÷ ÀÌ·¸°Ô ¾ò¾îÁø Á¤º¸¸¦ ÀÌ¿ëÇÏ¿© »ý¹°ÇÐÀû ½Ã½ºÅÛÀ» ¼öÁ¤¡¤º¸¿Ï¡¤Àç¼³°èÇÏ´Â 'Reverse engineering'±â¼ú°ú Á¢¸ñµÇ¾î ÃÖÀûÈ­µÈ Àΰø¹Ì»ý¹°À» Á¦ÀÛÇϴµ¥ ÇʼöÀûÀÎ ¹æ¹ýÀ¸·Î ÀÀ¿ëµÇ°í ÀÖ´Ù.

 

¡®Directed (molecular) evolution¡¯ ȤÀº 'in vitro evolution'Àº ÀüÇüÀûÀÎ evolutionary engineering ¹æ¹ýÀ¸·Î, random mutagenesis¿Í in vitro recombinationÀÇ ¹Ýº¹ÀûÀÎ °úÁ¤À» ÅëÇØ ºÐÀÚÀû ´Ù¾ç¼º À¯µµÇÏ¿© HTS (high-throughput screening or selection)·Î ƯÁ¤ Ç¥ÇöÇüÀ̳ª Çâ»óµÈ library member ¸¦ µ¿Á¤ÇÏ´Â Á¢±ÙÀÌ´Ù. ºñ±³Àû ªÀº ½Ã°£ ³»¿¡ ¿øÇϴ ǥÇöÇüÀ» ¾òÀ» ¼ö ÀÖ¾î º¸ÆíÀûÀ¸·Î ÀÌ¿ëµÇ´Âµ¥ error-prone PCR ±â¹ÝÀÇ mutagenesis, DNA shufflingÀÇ recombination µîÀÌ ¿©±â¿¡ ÇØ´çµÈ´Ù. ÇÏÁö¸¸, »èÁ¦º¯ÀÌ(deleterious mutations)ÀÇ ÃàÀû, ³·Àº ±³Â÷À²(crossover rate) µîÀÇ ¹®Á¦·Î ÀÎÇØ Á¦ÇÑµÈ °³Ã¼(population)¸¸ÀÌ ¼±º°µÇ¾î À̸¦ °³¼±Çϱâ À§ÇÑ ´Ù¾çÇÑ ¹æ¹ýµéÀÌ ½ÃµµµÇ°í ÀÖ´Ù. ¶ÇÇÑ ÀÌ´Â ÇÃ¶ó½º¹Ìµå »ó¿¡¼­ ¾ÆÁÖ ±ØÈ÷ Á¦ÇÑÀûÀÎ À¯ÀüÀÚ¸¸À» º¯ÀÌ ½Ãų ¼ö ÀÖ¾î ´ë»ç°æ·Î ¹× »ý¹°ÇÐÀû ½Ã½ºÅÛ µîÀÇ °³·®ÀÌ ¾Æ´Ñ ÀϹÝÀûÀ¸·Î È¿¼Ò °³·®¿¡ ÁÖ·Î È°¿ëµÇ°í ÀÖ´Ù.  


¡®Evolutionary adaptation¡¯ ȤÀº ¡®adaptative evolution'Àº ¡®directed evolution¡¯°ú´Â ´Þ¸® Áö³ð ¼öÁØ¿¡¼­ ±¤¹üÀ§ÇÏ°Ô µ¹¿¬º¯À̸¦ ÀÏÀ¸Å°°í À̵é Áß ÃÖÀûÈ­µÈ º¯ÀÌ ±ÕÁÖ¸¦ ºÐ¸®ÇÒ ¼ö ÀÖ¾î ÃÖ±Ù ÁÖ¸ñ¹Þ°í ÀÖ´Â ¹æ¹ýÀ¸·Î, ¹èÁö³ª ¹è¾çÁ¶°Ç µîÀÇ Æ¯Á¤ ȯ°æº¯È­¿¡ ÀûÀÀÇÏ¿© »ì¾Æ°¥ ¼ö ÀÖµµ·Ï ¸é¹ÐÈ÷ µðÀÚÀÎÇÏ¿© °íü ȤÀº ¾×ü¹è¾çÀ» ÅëÇØ ÀûÀÀµÈ ÁøÈ­°³Ã¼(evolved population)¸¦ ¼±º°ÇÏ´Â ¹æ¹ýÀÌ´Ù [±×¸² 1]. »ý¹°Ã¼ ÇÁ·Î¼¼½ºÀÇ º¹À⼺¿¡ ÀÇÇØ ½±°Ô ¾òÀ» ¼ö ¾ø´Â ´Ù¾çÇÑ Ç¥ÇöÇüÀ» ÀÌ·¯ÇÑ ¹æ¹ýÀ¸·Î ¾òÀ» ¼ö Àִµ¥ metabolic engineering ±â¼ú°ú ´õºÒ¾î À¯¿ë»ê¹°À» »ý»êÇÏ´Â »ê¾÷±ÕÁÖÀÇ °³¹ß¿¡ È¿°úÀûÀÎ ¹æ¹ýÀ¸·Î Á¦½ÃµÇ°í ÀÖ´Ù. ÃÖ±ÙÀÇ DNA sequencing ±â¼úÀÇ ¹ßÀüÀ¸·Î °ú°Å¿¡ ºñÇØ Ç¥ÇöÇüµéÀÇ À¯ÀüÁ¤º¸ ºÐ¼®ÀÌ ½±°Ô ÀÌ·ç¾îÁö´Âµ¥ ÀÌ·¯ÇÑ Ãß¼¼·Î ÀÎÇØ evolutionary adaptation ¹æ¹ýÀÌ ÇöÀç ¼±È£µÇ°í ÀÖ°í À̸¦ ÅëÇØ  ÃÖÀûÈ­µÈ µ¹¿¬º¯À̸¦ ºÐ¸®ÇÏ°í À̵éÀÇ Áö³ðºÐ¼®À¸·Î Ç¥ÇöÇüÀÇ À¯ÀüÁ¤º¸¿Í  À̸¦ È°¿ë°¡´ÉÇÏ°Ô µÇ¾ú´Ù.

±×¸² 1. Principle of evolutionary engineering [ref.1]


ÇÑÆí reverse engineering ¿ª½Ãµµ ÃÖ±Ù µé¾î °ü½ÉÀ» ¹Þ°í Àִµ¥ ÀÌ´Â °ú°Å °ªºñ½Ñ ºñ¿ë µîÀÇ Á¦ÇÑÀ¸·Î 'Forward engineering' ±â¹ÝÀÎ »õ·Î¿î »ý¹°ÇÐÀû part, device, systemÀÇ ±¸Ãà (design)¿¡¸¸ ±¹ÇѵÇÁö ¾Ê°í ÀÌÁ¦´Â 'Reverse engineering'Àº ¿À¹Í½º ±â¹ÝÀ¸·Î ƯÁ¤ ¹Ì»ý¹°ÀÇ Ç¥ÇöÇü¿¡ ´ëÇÑ Á¤È®ÇÑ ÀÌÇظ¦ ¹ÙÅÁÀ¸·Î »ý¹°ÇÐÀû ½Ã½ºÅÛÀÇ ÀçµðÀÚÀÎ (redesign) ÇÒ ¼ö ÀÖ´Â ¿µ¿ªÀ¸·Î±îÁö È®ÀåµÇ¾ú´Ù. ÀÌ·Î ÀÎÇØ ÀüÅëÀû À¯Àü°øÇбâ¼ú¿¡¼­ÀÇ ºÎºÐÀûÀÌ°í Á¦ÇÑÀûÀÎ »ý¸íÁ¤º¸¸¦ ³Ñ¾î »ý¸í½Ã½ºÅÛÀÇ Àü¹ÝÀûÀÎ ÀÌÇظ¦ Åä´ë·Î º¸´Ù È¿À²Àû¡¤Ã¼°èÀûÀ¸·Î »ý¸íü¿¡ ´ëÇÑ Á¢±ÙÀÌ °¡´ÉÇÏ°Ô µÇ¾ú´Ù [±×¸² 2]. 

±×¸² 2. Synergy between Synthetic and Systems Biology [ref. 3]

     

ÇöÀç ÇÕ¼º»ý¹°ÇÐ ºÐ¾ß¿¡¼­ ¹Ì»ý¹° À¯·¡ »ê¾÷Àû ¹ÙÀÌ¿ÀÈ­ÇÐÁ¦Ç°»ý»êÀ» À§ÇÑ °¡Àå Å« È­µÎ Áß Çϳª°¡ ¹Ù·Î °í¼º´É ¸ÂÃãÇü »ê¾÷Àû »ý»ê±ÕÁÖÀÇ °³¹ßÀÌ´Ù. ÇöÀç »ê¾÷±ÕÁÖ°³¹ßÀ» À§ÇÑ ÀÏ·ÃÀÇ ³ë·Âµé·Î ź¼Ò¿ø À̿뼺ÀÇ Çâ»ó (improved carbon source utilization), »ý»ê¼º Áõ°¡ (increased product formation), ½ºÆ®·¹½º ÀúÇ×¼º (stress tolerance)À» ȹ±âÀûÀ¸·Î ´Ã¸®±â À§ÇÑ ½Ãµµ°¡ ÀÌ·ç¾îÁö°í ÀÖÀ¸¸ç, ƯÁ¤ phenotype »ý¼º¿¡ ÀÖ¾î ±âÁ¸ ±â¼úÀÎ mutagenesis, screening, evolutionary engineering µî°ú ÇÔ²² ÃÖ±ÙÀÇ reverse engineeringÀº »ê¾÷Àû »ý¹°°øÇко߿¡¼­ ¹Ì»ý¹° ´ë»çȸ·ÎÀÇ ÃÖÀûÈ­ ºÐ¼®¿¡ Áß¿äÇÑ ±â¹ýÀ¸·Î ÀÚ¸®¸Å±èÇÏ°í ÀÖ´Ù [±×¸² 3]. »ý¹°Ã¼ ÇÁ·Î¼¼½ºÀÇ º¹À⼺À¸·Î ÀÎÇØ »ý¹°Ã¼¿¡ ´ëÇÑ ¿Ïº®ÇÑ Çؼ®Àº Èûµç ½ÇÁ¤ÀÌÁö¸¸ reverse engineering, DNA ÇÕ¼º ¹× ½ÃÄö½Ì ±â¼úÀÇ ¹ßÀüÀº º¸´Ù ±¸Ã¼ÀûÀÌ°í ¸¹Àº »ý¸íÇö»óÀÇ ºÐ¼®°ú ¿¹ÃøÀ» °¡´ÉÇÏ°Ô ÇØÁÖ´Â °ÍÀº ºÎÀÎÇÒ ¼ö ¾ø´Ù. 


¾ÕÀ¸·Î ¹Ì·¡»ê¾÷À¸·Î¼­ÀÇ ÀáÀ缺ÀÌ Å« ¹ÙÀÌ¿ÀÈ­Çлê¾÷ÀÇ ¼ºÆд ÃÖÁ¾»ê¹°À» °æÁ¦¼º ÀÖ°í ºü¸£°Ô »ý»êÇÏ´Â °ÍÀÌ Áß¿äÇϸç À̸¦ À§Çؼ­´Â °í¼º´É Áö´ÉÇü/¸ÂÃãÇü Àΰø»ý¸íü °³¹ßÀÌ ÇʼöÀûÀÌ´Ù. ÀÌ¿¡ ÃÖ±ÙÀÇ º» ¿¬±¸½Ç¿¡¼­ °³¹ßµÈ Áö³ð¿£Áö´Ï¾î¸µ Àû¿ë ±â¼úÀ» ¼Ò°³ÇÏ°íÀÚ ÇÑ´Ù. ÀÌ´Â Áö³ð ¼öÁØ¿¡¼­ µ¹¿¬º¯À̸¦ ºü¸¥ ¼Óµµ·Î Á¦ÀÛÇÒ ¼ö ÀÖ¾î ´ë»ç°æ·Î¸¦ ÃÖÀûÈ­Çϱâ À§ÇØ ¾ÆÁÖ ¸¹Àº °æ¿ìÀÇ ¼ö¸¦ Á¦ÀÛÇÏ°í À̵é Áß¿¡¼­ ÃÖÀû º¯ÀÌ ±ÕÁÖ¸¦ ºü¸£°Ô ¼±º°ÇÒ ¼ö ÀÖ´Ù. º» ¹æ¹ýÀº evolutionary adaptationÀÌ °¡Áö°í ÀÖ´Â ¹®Á¦Á¡ (±ä ½Ã°£ ¿ä¼Ò, ¹«ÀÛÀ§ µ¹¿¬º¯ÀÌ, ³·Àº º¯ÀÌÀ², µî)À» ±Øº¹ÇÒ ¼ö ÀÖ¾î ¸ÂÃãÇü Àΰø »ý¸íü Á¦ÀÛ¿¡ ¾ÆÁÖ À¯¿ëÇÏ°Ô È°¿ëµÉ °ÍÀÌ´Ù.

±×¸² 3. Optimization of microbial metabolic pathways in

industrial biotechnology [ref. 4]


ÃÖ±ÙÀÇ Áö³ð¿£Áö´Ï¾î¸µ ±â¹ýµéÀº ÇÕ¸®ÀûÀÎ À¯ÀüüÀÇ ÀÌÇظ¦ ¹ÙÅÁÀ¸·Î ÇÑ À¯ÀüÀÚ ÁöµµÀÇ ¼³°è¿Í ÇÕ¼ºÀ» ÅëÇØ ´ë»ç»ê¹°ÀÇ »ý»ê·®À» Áõ°¡½Ãų ¼ö ÀÖ´Â ¹æÇâÀ¸·Î °³¹ßµÇ°í Àִµ¥ À̸¦ ÅëÇØ ´Ù¾çÇÑ Áö´ÉÇü Àΰø »ý¸íü¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ±â¹ÝÀ» Á¦°øÇØ ÁÖ°í ÀÖ´Ù. ÀÌ·¯ÇÑ ±â¼ú°¡¿îµ¥ MAGE (multiplex automated genome engineering)¶ó°í ¾Ë·ÁÁø ±â¼úÀº ssDNA¸¦ ¼¼Æ÷³»·Î µµÀÔ½ÃÅ´À¸·Î½á ¾ÆÁÖ °£´ÜÇÏ°Ô ´Ù¼öÀÇ µ¹¿¬º¯À̸¦ ºñ±³Àû ´Ü½Ã°£¿¡ Á¦ÀÛÇÒ ¼ö ÀÖ°Ô ÇØÁØ´Ù.

 

MAGE´Â mismatch repair¿¡ °ü¿©ÇÏ´Â mutS À¯ÀüÀÚ Á¦°Å½ÃÅ´°ú µ¿½Ã¿¡ ¥ë-red systemÀÇ ¹ßÇöÀ» ÅëÇØ ssDNA »ðÀÔÀ» 1000¹è±îÁöµµ Áõ°¡½Ãų ¼ö ÀÖ´Ù. MAGE¿Í °°ÀÌ ssDNA-mediated recombinationÀÇ °æ¿ì ´Ù¸¥ plasmid³ª dsDNAº¸´Ù À¯ÀüÀÚ µµÀÔ(transformation)È®·üÀÌ ³ô±â ¶§¹®¿¡ º¸´Ù ³ôÀº È¿À²ÀÇ À¯ÀüÀÚ ÀçÁ¶ÇÕÀ» ±â´ëÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó ÇÑ cycle´ç ¼Ò¿äµÇ´Â ½Ã°£ÀÌ »ó´ëÀûÀ¸·Î ´Ù¸¥ ±â¹ýµéº¸´Ù ª¾Æ ¸¹Àº ¼öÀÇ µ¹¿¬º¯ÀÌ ±ÕÁÖ¸¦ ¸¸µé ¼ö ÀÖ´Ù. ÀÌ·¯ÇÑ Áö³ð¿£Áö´Ï¾î¸µÀ» Á¢¸ñÇÑ ÇÕ¼º»ý¹°Çаú ´ë»ç°øÇÐÀº lycopene, squaleneÀÇ ±â´É¼º ½ÄÇ° ȤÀº ÀǾàÇ° µîÀÇ À¯¿ë»ê¹° »ý»êÀÌ °¡´ÉÇÑ ´Ù¾çÇÑ platform ±ÕÁÖ°³¹ßÀ» °¡´ÉÇÏ°Ô ÇÑ´Ù. ±×·¯³ª ±âÁ¸ ±â¼ú¿¡¼­´Â ƯÁ¤ E. coli ±ÕÁÖ¸¸ÀÌ ÀÌ¿ëµÉ ¼ö ÀÖ¾î º¸´Ù ´Ù¾çÇÑ ´ëÀå±Õ ±ÕÁÖ·ÎÀÇ È®À强ÀÌ ÇöÀúÈ÷ ¶³¾îÁö°í ÀÖ´Â ½ÇÁ¤ÀÌ´Ù. ƯÈ÷ À¯¿ë»ê¹°ÀÇ »ê¾÷Àû »ý»êÀ» À§Çؼ­´Â ´Ù¾çÇÑ ±ÕÁÖ»Ó¸¸ ¾Æ´Ï¶ó »ê¾÷¿ë ´ëÀå±ÕÀ¸·ÎÀÇ ¹ü¿ëÀûÀÎ ±â¼úÀÌ ²À ÇÊ¿äÇÏ´Ù. ÀÌ¿¡ º» ¿¬±¸ÆÀÀº ´Ù¾çÇÑ E. coli¿¡¼­µµ MAGE¸¦ È®Àå °¡´ÉÇϵµ·Ï ÇÒ ¼ö ÀÖ´Â ¡®pRED suicide plasmids¡¯ ±â¹ÝÀÇ »õ·Î¿î ±â¼úÀ» ÃÖ±Ù °³¹ßÇÏ¿´´Ù [±×¸² 4].


±×¸² 4. The  Portable Lambda-Red System (PLRS)

 

 

2. ¿¬±¸°á°ú
 • º» ¿¬±¸¿¡¼­´Â portable pRED Çö󽺹̵åÀÇ mutS À¯ÀüÀÚ·ÎÀÇ »ðÀÔÀ¸·Î ´Ù¾çÇÑ ±ÕÁÖ¿¡¼­ Áö³ð
  ¿£Áö´Ï¾î¸µ Àû¿ëÀ»          °¡´ÉÇÏ°Ô ÇÏ¿´´Âµ¥, pINZ Çö󽺹̵忡 Ŭ·Î´×µÈ ÀÌÁ¾ À¯ÀüÀÚÀÇ ´ëÀå±Õ Áö³ð»ðÀÔ ÈÄ Áö³ð¿£Áö´Ï¾î¸µÀ» ÀÌ¿ëÇÏ¿© ¹ßÇö        ÃÖÀûÈ­¸¦ À¯µµÇÒ ¼ö ÀÖµµ·Ï Portable Lamda Red System (PLRS)À» ±¸ÃàÇÏ¿´´Ù [±×¸² 4].
 • ¹ü¿ë ±ÕÁÖ¿¡¼­ MAGE°¡ °¡´ÉÇϵµ·Ï °³¹ßµÈ Áö³ð±â¹Ý Áö³ð¿£Áö´Ï¾î¸µ Àû¿ë±â¼úÀ» ÀÌ¿ëÇÏ¿© 
È¿À²ÀûÀ¸·Î ´Ù¾çÇÑ lycopene »ýÇÕ¼º º¯ÀÌ´ëÀå±Õ »ý¼ºµÇ¾î À̸¦ ½ºÅ©¸®´×ÇÏ¿© ´ëÀå±Õ¿¡¼­ lycopene »ýÇÕ¼ºÀÌ ¼º°øÀûÀ¸·Î º¯È­µÊÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù     [±×¸² 5]. »õ·Ó°Ô ¾ò¾îÁø ±ÕÁÖµéÀÇ re-sequencingÀ» ÅëÇØ lycopene »ýÇÕ¼º¿¡¼­ Áß¿äÇÑ ¿ªÇÒÀ» ÇÏ´Â dxs ¿Í ipiÀÇ ¹ßÇö           °­È­¿Í °ü·ÃµÈ ´Ù¾çÇÑ ¿°±â¼­¿­ÀÇ RBS¸¦ È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù.

 • ÀÌó·³ º» ¿¬±¸¸¦ ÅëÇØ À¯ÀüÀÚ ÀçÁ¶ÇÕ È¿¼Ò¸¦ ¹ßÇöÇÏ´Â Çö󽺹̵åÀÇ Áö³ð»ó mutS À¯ÀüÀÚ·ÎÀÇ »ðÀÔÀ» ÅëÇØ ¹ü¿ëÀûÀΠ    ´ëÀå±Õ Áö³ð¿£Áö´Ï¾î¸µ Àû¿ë ±â¼úÀ» ±¸ÃàÇÏ¿© ¿©·¯ ÀÌÁ¾ À¯ÀüÀÚµéÀÇ ´ëÀå±Õ Áö³ð»óÀÇ µµÀÔÀ» ¿ëÀÌÇÏ°í ¼±º°ÇÒ ¼ö ÀÖ°Ô µµ¿ÍÁÖ´Â ÀÚ»ì Çö󽺹̵å Á¦ÀÛÀ» ÇÏ¿´´Ù.

    ¶ÇÇÑ, º» °³¹ßµÈ ±â¼úÀ» ´ëÀå±Õ³»ÀÇ lycopene »ýÇÕ¼º ´ë»ç°æ·Î¸¦ ÃÖÀûÈ­Çϴµ¥ Àû¿ëÇÔÀ¸·Î½á  ¹ÙÀÌ¿ÀÈ­ÇÐ »ê¾÷¿ë ´Ù¾çÇÑ Àΰø ´ëÀå±Õ °³¹ß ±â¼úÀÓÀ» ÀÔÁõÇϴµ¥ ¼º°øÇÏ¿´´Ù.
 • º» ±â¼úÀº ´Ù¾çÇÑ À¯¿ë¹°Áú »ýÇÕ¼º ´ë»ç°æ·Î ÃÖÀûÈ­¿¡ Àû¿ë °¡´ÉÇÏ¿© ¹Ì»ý¹° ±â¹Ý ¹ÙÀÌ¿ÀÈ­ÇР
»ê¾÷¿¡ °ü·ÃµÈ »ýÇÕ¼º     ´ë»ç°æ·Î ÀÀ¿ëÀ¸·Î È®ÀåÀÌ µÉ ¼ö ÀÖÀ¸¸ç ¹ÙÀÌ¿À»ê¾÷¿ë Ç÷§Æû Àΰø ´ëÀå±Õ Á¦ÀÛ¿¡ ÇÊ¿äÇÑ Áö³ð ¼öÁØÀÇ ´Ù¾çÇÑ ÇÕ¼º»ý¹°ÇÐ ±â¼ú °³¹ß°ú À̸¦ È°¿ëÇÑ ¸ÂÃãÇü Àΰø ¹Ì»ý¹° Á¦ÀÛ¿¡ ±¤¹üÀ§ÇÑ ½Ã³ÊÁö È¿°ú âÃâÀ» ±â´ëÇÏ°í ÀÖ´Ù.

±×¸² 5. PLRS system

 

3. °á·Ð

º» ¿¬±¸°³¹ßÀº »ý¸í°øÇÐ Á¦Ç°ÀÇ »ý»ê¼ÓµµÀÇ Áõ´ë»Ó¸¸ ¾Æ´Ï¶ó ¼öÀ²ÀÇ Áõ´ë, ±×¸®°í ½Å¹°Áú °³¹ß µîÀ» À§ÇÑ ÇʼöÀûÀÎ ±â¼ú·Î ÀÚ¸®¸Å±èÇÒ °ÍÀ¸·Î ¿¹»óµÈ´Ù. À̸¦ ±â¹ÝÀ¸·Î ´Ù¾çÇÑ ¹ÙÀÌ¿À È­Çй°Áú »ýÇÕ¼º ÃÖÀûÈ­ ´ë»ç°æ·Î Á¦ÀÛ ¹× µµÀÔÀ¸·Î »ý»ê´Ü°¡ Àú°¨, »ý»ê¼ÓµµÀÇ Áõ´ë»Ó¸¸ ¾Æ´Ï¶ó ¼öÀ²ÀÇ Áõ´ë, ±×¸®°í ½Å¹°Áú °³¹ß µîÀ» À§ÇÑ ÇÙ½ÉÀû ±â¼ú·Î ÀÚ¸®¸Å±èÇÑ´Ù¸é ¹ÙÀÌ¿ÀÀÌÄÚ³ë¹Ì ½Ã´ë¸¦ ÁغñÇϴµ¥ ÇâÈÄ °æÁ¦Àû »ê¾÷Àû Æı޷ÂÀº ³ôÀ» °ÍÀ¸·Î ¿¹»óµÈ´Ù. ÇöÀç ¼¼°è °¢±¹¿¡¼­ ´ë»ç°øÇÐÀûÀÎ ±â¼ú¹æ¹ýÀ» ÅëÇÏ¿© ±ÕÁÖ¸¦ °³·®ÇÏ·Á´Â ½Ãµµ´Â ´Ù¾çÇÑ Ãø¸é¿¡¼­ ÀÌ·ç¾îÁö°í ÀÖ´Ù. ÀÌµé °³·®µÈ ±ÕÁÖµéÀº È­ÇÐ(Á¤¹ÐÈ­Çй°Áú, ¹ü¿ëÈ­Çй°Áú), ÀǾà, ÀÇ·á(±¤ÇÐÈ°¼º ÀǾàÇ°, ½Å±ÔÇ×»ýÁ¦), ȯ°æ(³­ºÐÇؼº È­ÇÕ¹° ºÐÇØ, »ýºÐÇؼº °íºÐÀÚ), ³ó¾÷(Áú¼Ò°íÁ¤È­, ÇØÃæ³»¼º) µî ´Ù¾çÇÑ »ê¾÷ ºÐ¾ß¿¡ ÀÀ¿ëµÇ°í ÀÖ´Ù. ÃÖ½ÅÀÇ ¿¬±¸°³¹ß °æÇâÀÎ ½Ã½ºÅÛ»ý¹°°øÇÐ ¹× ÇÕ¼º»ý¹°ÇÐÀÇ ¹ß´Þ·Î ÀÎÇÏ¿© ±ÕÁÖ¸¦ ºÎºÐÀûÀÌ ¾Æ´Ï¶ó ÀüüÀûÀ¸·Î ÀÌÇØÇÏ°í óÀ½ºÎÅÍ ¿¹Ãø °¡´ÉÇÑ Á¶Àý±âÀÛµéÀ» ½×¾Æ¿Ã·Á »õ·Î¿î ±ÕÁÖ¸¦ °³¹ßÇÏ´Â ÇÑ Â÷¿ø ³ôÀº ´ë»ç ÃÖÀûÈ­ ±â¼úµéÀÌ È°¹ßÈ÷ ½ÃµµµÉ °ÍÀ¸·Î ¿¹»óµÇ¸ç ¿ì¸®µµ ÀÌ¿¡ ¹ß¸ÂÃç ´Ù°¡¿À´Â ¹Ì·¡ »ý¹°°æÁ¦»çȸ (Biobased economy)¸¦ ÁغñÇØ¾ß µÈ´Ù. º» ¿¬±¸ÆÀ¿¡¼­´Â [±×¸² 6]¿¡¼­¿Í °°ÀÌ ´Ù¾çÇÑ ÇÕ¼º»ý¹°ÇÐ ±â¼úÀ» °³¹ßÇÏ°í À̸¦ È°¿ëÇÏ¿© ¹ÙÀÌ¿À»ê¾÷È­ ÀÀ¿ëÀ» À§ÇÑ Àΰø¹Ì»ý¹° °³¹ß¿¡ ³ë·ÂÇÏ°í ÀÖ´Ù.

±×¸² 6. Development of synthetic microbes based key biotechnologies

 

4. Âü°í¹®Çå

1. Hahn-Hägerdal et al. (2005) Role of cultivation media in the development of yeast strains for

    large scale industrial use. Microbial Cell Factories 4:31
2. Stephen Lutz, (2010) Beyond directed evolution- semi-rational protein engineering and

    design, Curr Opin Biotechnol. 21: 734–743.
3. Federici F, Rudge TJ, Pollak B, Haseloff J, Gutiérrez RA., (2013) Synthetic Biology:

    opportunities for Chilean bioindustry and education. Biol Res. 46, 383-93.
4. Herrgård, M., Panagiotou, G. 2012. Analyzing the genomic variation of microbial cell factories

    in the era of ¡°New Biotechnology¡±.  Comput Struct Biotechnol J. 3, e201210012.
5. Ryu Ys, Biswas RK, Shin K, Parisutham V, Kim SM, and Lee SK. (2014) A simple and

    effective method for construction of Escherichia coli strains proficient for genome

    engineering. PLoS ONE 9: e94266
6. Mosberg JA, Gregg CJ, Lajoie MJ, Wang HH, Church GM (2012) Improving lambda Red

    genome engineering in Escherichia coli via rational removal of endogenous nucleases.

    PLoS One 7: e44638.
7. Esvelt K, Wang HH (2013) Genome-scale engineering for systems and synthetic biology. Mol

    Syst Biol 9: 641.
8. Wang HH, Isaacs FJ, Carr PA, Sun ZZ, Xu G, et al. (2009) Programming cells by multiplex

    genome engineering and accelerated evolution. Nature 460: 894–898.
9. Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, et al. (2011) Precise manipulation of

    chromosomes in vivo enables genome-wide codon replacement. Science 333: 348–353.
10. Sandoval NR, Kim JYH, Glebes TY, Reeder PJ, Aucoin HR, et al. (2012) Strategy for directing

      combinatorial genome engineering in Escherichia coli. Proc Natl Acad Sci USA 109:

      10540–10545.
11. Yu DG, Ellis HM, Lee EC, Jenkins NA, Copeland NG, et al. (2000) An efficient recombination

      system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci USA 97:

      5978–5983.
12. Datta S, Costantino N, Court DL (2006) A set of recombineering plasmids for Gram-

     negative bacteria. Gene 379: 109–115.

 




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