¿ÞÂÊ ¸Þ´º ŸÀÌƲ À̹ÌÁö
Áֿ伺°ú

Home < Áֿ伺°ú < Áֿ伺°ú

       Ç×±Õ ÆéŸÀ̵å ÁßÇÕüÀÇ ´ëÀå±Õ Ç¥¸é ¹ßÇö ¹× ÀÌÀÇ Ç×»ýÁ¦ ´ëüÀç·ÎÀÇ Àû¿ë
               
       ISBC        2018.08.20 16:19        3243
 

Ç×±Õ ÆéŸÀ̵å ÁßÇÕüÀÇ ´ëÀå±Õ Ç¥¸é ¹ßÇö ¹× ÀÌÀÇ Ç×»ýÁ¦ ´ëüÀç·ÎÀÇ Àû¿ë



Áö´ÉÇü ¹ÙÀÌ¿À½Ã½ºÅÛ ¼³°è ¹× ÇÕ¼º ¿¬±¸´Ü ½ÅÁÖ¸® ¿¬±¸¿ø



Display of multimeric antimicrobial peptides on the Escherichia coli cell surface and its application as whole-cell antibiotics / PLoS One. 2013;8(3):e58997.


1. ¿¬±¸¹è°æ


º´¿ø±ÕÀ¸·ÎºÎÅÍ Àηù¸¦ º¸È£Çϱâ À§ÇØ ¸¹Àº Ç×»ýÁ¦µéÀÌ ¹ß°ß ¹× °³¹ßµÇ¾î »ç¿ëµÇ¾î ¿ÔÀ¸³ª, Ç×»ýÁ¦ÀÇ ¿À³²¿ëÀÇ °á°ú·Î ¿ÀÈ÷·Á Ç×»ýÁ¦ ³»¼º ±ÕÁÖµéÀÌ ±Þ¼ÓÀÌ ´Ã¾î³ª°Ô µÊ¿¡ µû¶ó »ç¿ë °¡´ÉÇÑ Ç×»ýÁ¦ÀÇ ¼ö°¡ ÁÙ¾îµé°í ÀÖ´Â ¹®Á¦°¡ ¹ß»ýÇÏ¿´´Ù. ƯÈ÷ °¡Ãà¿¡¼­ÀÇ ¹«ºÐº°ÇÑ Ç×»ýÁ¦ »ç¿ëÀº Ç×»ýÁ¦ ³»¼º±Õ È®»êÀÇ ÁÖ¿ä ¿øÀÎÀÌ µÇ¾ú°í, ÀÌµé ³»¼º±Õ¿¡ »ç¶÷ÀÌ °¨¿°µÇ¾úÀ» ¶§ »ç¿ëÇÒ ¼ö ÀÖ´Â Ç×»ýÁ¦ÀÇ ¼ö°¡ ±Þ°ÝÈ÷ ÁÙ¾îµé¸é¼­ Àü ¼¼°èÀûÀ¸·Î °¡Ãà¿¡ »ç¿ëÇÏ´Â Àμö°øÅë Ç×»ýÁ¦ÀÇ »ç¿ëÀ» Àü¸é ±ÝÁöÇÏ°í ÀÖ´Â Ãß¼¼ÀÌ´Ù (1-4). ÀÌ¿¡ ±âÁ¸ÀÇ Ç×»ýÁ¦¸¦ ´ëü ÇÒ ¼ö ÀÖ´Â ¹°ÁúÀÌ ¿¬±¸µÇ°í ÀÖ´Â °¡¿îµ¥ Ç×±Õ ÆéŸÀ̵尡 ±× ´ë¾ÈÀ¸·Î ¿©°ÜÁö°í ÀÖ´Ù (5). Ç×±Õ ÆéŸÀ̵å´Â ±âÁ¸ÀÇ Ç×»ýÁ¦¿Í´Â ´Þ¸® ±¤¹üÀ§ÇÑ ¹Ì»ý¹°¿¡ ´ëÇÏ¿© °­·ÂÇÑ Ç×±Õ·ÂÀ» °¡Áö¸ç, ¿­, »ê ¶Ç´Â ¾ËÄ®¸® µî¿¡ °­ÇÑ ÀÌÈ­ÇÐÀû ¾ÈÁ¤¼ºÀ» °¡Áö°í, ¾Æ¹Ì³ë»êÀÌ 5 ³»Áö 50°³·Î ÀûÀº ¼öÀÇ ¾Æ¹Ì³ë»êÀ¸·Î ÀÌ·ç¾îÁ® Àֱ⠶§¹®¿¡ Ç×±Õ ÀÛ¿ë ÈÄ ½±°Ô ºÐÇصǾî ü³»¿¡ ÀÜ·ùÇÏÁö ¾Ê¾Æ »ýü ³» µ¶¼ºÀ» À¯¹ßÇÏÁö ¾Ê´Â µîÀÇ ¿©·¯ °¡Áö ÀåÁ¡À» °¡Áö°í ÀÖ´Ù. µû¶ó¼­ Ç×±Õ ÆéŸÀ̵å´Â Â÷¼¼´ë Ç×»ý ¹°Áú·Î ÀÌ¿ë °¡Ä¡°¡ ¸Å¿ì ³ô°í, »ê¾÷Àû ÀÀ¿ë ¿ä±¸µÇÁö¸¸ ±âÁ¸ÀÇ ÆéŸÀÌµå »ý»ê ¹æ¹ýÀ¸·Î´Â Ç×±Õ ÆéŸÀ̵带 °ª½Î°Ô ´ë·®À¸·Î °ø±ÞÇÒ ¼ö ¾ø´Ù. Áï, ´ëÇ¥ÀûÀÎ ÆéŸÀÌµå »ý»ê¹ýÀÎ È­ÇÐÇÕ¼ºÀ» ÀÌ¿ëÇÏ´Â °æ¿ì¿¡´Â °æÁ¦¼ºÀÌ ³·°í, »ý¹°ÇÐÀûÀ¸·Î ¹Ì»ý¹°À» ÅëÇØ »ý»ê ÇÒ °æ¿ì¿¡´Â Ç×±Õ ÆéŸÀ̵åÀÇ ¹ßÇöÀ²ÀÌ ³·°í ¼÷ÁÖ¿¡ ´ëÇÑ Ç×±Õ È°¼ºÀ» ³ªÅ¸³¾ »Ó¸¸ ¾Æ´Ï¶ó ¹ßÇöµÈ Ç×±Õ ÆéŸÀ̵尡 ¼÷ÁÖ ³» ´Ü¹éÁú ºÐÇØÈ¿¼Ò·ÎºÎÅÍ ºÐÇصȴٴ ¹®Á¦Á¡ÀÌ ÀÖ´Ù (6).


µû¶ó¼­ º» ¿¬±¸¿¡¼­´Â Ç×±Õ ÆéŸÀ̵带 °¡Ãà¿¡ »ç¿ëµÉ ¼ö ÀÖÀ» Á¤µµÀÇ °æÁ¦¼ºÀ» °¡Áö¸ç ´ë·® °ø±ÞÀÌ °¡´ÉÇÑ ¹æ¹ýÀ» °í¾ÈÇÏ¿´´Ù. ¸ÕÀú Æé½Å¿¡ ÀÇÇØ Àý´ÜµÇ´Â ´Ü·®Ã¼·Î ÀÌ·ç¾îÁø Ç×±Õ ÆéŸÀÌµå ´ÙÁßÇÕü¸¦ ¹ßÇöÇÏ´Â ¹Ì»ý¹°À» ±¸ÃàÇÏ°í À̸¦ »ý±Õ »óÅ·Π»ç¿ëÇÏ¿© ºÐ¸® ¹× Á¤Á¦°úÁ¤ ¾øÀÌ »ýü ³»¿¡¼­ Ç×±Õ È°¼ºÀ» °¡Áöµµ·Ï ÇÏ¿´´Ù (±×¸². 1).


±×¸² 1. ¼¼Æ÷ Ç¥¸é¿¡ Ç×±Õ ÆéŸÀÌµå ´ÙÁßÇÕü¸¦ ¹ßÇöÇÏ´Â Ç×»ý¹Ì»ý¹°°ú ÀÛ¿ë ±âÀÛ.

(A) Æé½Å¿¡ ÀÇÇØ ´Ü·®Ã¼·Î ºÐÇصǴ Ç×±Õ ÆéŸÀÌµå ´ÙÁßÇÕü¸¦ ¼¼Æ÷ Ç¥¸é¿¡ ¹ßÇöÇÏ´Â Ç×»ý¹Ì»ý¹°ÀÇ °³¿äµµ (B) °æ±¸Åõ¿©¸¦ ÅëÇØ Ç×»ý ¹Ì»ý¹°ÀÌ Ã¼³»¿¡ µé¾î¿À¸é À§ ¼ÓÀÇ Æé½Å¿¡ ÀÇÇØ Ç×±Õ ÆéŸÀÌµå ´ÙÁßÇÕü°¡ È°¼ºÀ» °¡Áö´Â ´Ü·®Ã¼·Î Àß·Á³ª¿È (C) È°¼ºÀ» °¡Áö´Â Ç×±Õ ÆéŸÀÌµå ´Ü·®Ã¼´Â ÁÖÀ§ÀÇ º´¿ø±ÕÀ» °ø°ÝÇÏ¿© À̵éÀÇ »ýÀåÀ» ¾ïÁ¦ÇÔ



2. ¿¬±¸°á°ú


¸ÕÀú ³ôÀº Ç×±ÕÈ°¼º°ú Æé½Å¿¡ ÀÇÇØ ³»ºÎ°¡ À߸®Áö ¾Ê°í, °í³óµµ¿¡¼­ ¼¼Æ÷ µ¶¼ºÀÌ ¾ø´Â Buf IIIb (RVVRQWPIGRVVRRVVRRVVR) Ç×±Õ ÆéŸÀ̵带 »ç¿ëÇÏ¿© Æé½Å¿¡ ÀÇÇØ À߸®µµ·Ï ´Ü·®Ã¼ÀÇ c-¸»´Ü¿¡ ·ù½ÅÀ» ÷°¡ÇÏ¿© ÁßÇÕü¸¦ Á¦ÀÛÇÏ°í, Lpp-OmpA anchor proteinÀ» »ç¿ëÇÏ¿© ´ëÀå±Õ¿¡ Ç¥¸é¿¡ Ç×±Õ ÆéŸÀ̵å ÁßÇÕü(Lpp-OmpA-multimeric Buf IIIb-Ln(LO-Bn))°¡ ¹ßÇöÇϵµ·Ï ÇÏ¿´´Ù (7-8), (±×¸². 2). ±× °á°ú Ç×±Õ ÆéŸÀ̵å ÁßÇÕü°¡ ¼¼Æ÷Ç¥¸é¿¡ ¹ßÇöµÊÀ» È®ÀÎÇÏ¿´°í, Ç×±Õ ÆéŸÀ̵å ÁßÇÕüÀÇ ±æÀÌ°¡ ±æ¾îÁú¼ö·Ï ¼¼Æ÷Ç¥¸é ¹ßÇöÀÌ ¸¹ÀÌ µÇ¸ç ´õ ¸¹Àº ¾çÀÇ ÀçÁ¶ÇÕ Ç×±Õ ÆéŸÀ̵å Buf IIIb-LÀ» ¾òÀ» ¼ö ÀÖ¾ú´Ù (±×¸². 3). À̸¦ ½ÇÁ¦ Àû¿ëÀ» °¡Á¤ÇÏ¿© ±ÕÁÖ ÀÚü¸¦ Æé½Å ó¸® ÇÑ µÚ ¾ò¾îÁø extract¸¦ »ç¿ëÇÏ¿© °¡Ãà Áúº´ÀÇ ÁÖ¿ä ¿øÀÎ ±ÕÀÎ Salmonella enteritidis ¿ÍListeria monocytogenes¿¡ ´ëÇØ Ç×±Õ·ÂÀ» ÃøÁ¤ÇÑ °á°ú ¼¼Æ÷Ç¥¸é¿¡ °¡Àå ¸¹ÀÌ ¹ßÇöµÇ¾ú´ø LO-B©ýÀÇ È°¼ºÀÌ °¡Àå ÁÁÀ½À» È®ÀÎÇÏ¿´´Ù (±×¸². 4). In vitro ½ÇÇè °á°ú¿Í ¸¶Âù °¡Áö·Î mouse¸¦ ÀÌ¿ëÇÑ in vivo ½ÇÇè¿¡¼­µµ LO-B©ý¸¦ ¹ßÇöÇÏ´Â ´ëÀå±ÕÀÇ È°¼ºÀÌ LO-B©ûÀÇ È°¼ºº¸´Ù ³ôÀ½À» È®ÀÎ ÇÒ ¼ö ÀÖ¾ú´Ù.



±×¸² 2. LO-Bn À¯ÀüÀÚ¿Í LO-Bn fusion proteinsÀÇ ¹ßÇö È®ÀÎ. (A) NotIÀ¸·Î ÀÚ¸¥ LO-Bn DNAÀýÆí (B) SDS-PAGE·Î È®ÀÎÇÑ LO-Bn fusion proteinsÀÇ ¹ßÇö



±×¸² 3. ¼¼Æ÷ Ç¥¸é¿¡ ¹ßÇöµÇ´Â LO-Bn fusion proteinsÀÇ È®ÀÎ.

(A) ¼¼Æ÷ Ç¥¸é¿¡ Ç×±Õ ÆéŸÀ̵å ÁßÇÕü¸¦ ¹ßÇöÇÏ´Â ´ëÀå±ÕÀÇ confocal images. (B) LO-BnÀÇ ¼¼Æ÷ Ç¥¸é ¹ßÇö¾ç. (C) LO-Bn À» ¹ßÇöÇÏ´Â ´ëÀå±Õ 1 x 10©ö©ö À» ÀÌ¿ëÇÏ¿© ¾òÀº LO-Bn°ú ÀçÁ¶ÇÕ Buf IIIb-LÀÇ ¾ç



±×¸² 4. LO-B©û°ú LO-B©ýÀ» ¹ßÇöÇÏ´Â ´ëÀå±ÕÀÇ Æé½Å ó¸® ÈÄ Ç×±Õ È°¼º


3. ¿¬±¸ÀÇ ¼º°ú ¹× ÀÇÀÇ


Ç×±Õ ÆéŸÀ̵å´Â ±Þ¼ÓÇÏ°Ô ÆÛÁö°í ÀÖ´Â Ç×»ýÁ¦ ³»¼º¹®Á¦ÀÇ ÇØ°áÃ¥À¸·Î °¡Ãà ¹× »ç¶÷¿¡°Ô »ç¿ëÇÒ ¼ö ÀÖ¾î Á߿伺ÀÌ ºÎ°¢µÇ°í ÀÖÁö¸¸, °¡Ãà»ê¾÷¿¡¼­´Â Áö³ªÄ¡°Ô ºñ½Ñ °¡°ÝÀ¸·Î ´Ù·®ÀÇ Ç×±Õ ÆéŸÀ̵带 Àû¿ëÇÒ ¼ö ÀÖ´Â ¹æ¹ýÀÌ Àü¹«ÇÏ¿´´Ù. ±×·¯³ª º» ¿¬±¸¿¡¼­´Â °í°¡ÀÇ Ç×±Õ ÆéŸÀ̵带 »ý»êÇϱâ À§ÇÏ¿© ¼¼Æ÷ Æļâ, ºÐ¸® ¹× Á¤Á¦°úÁ¤À» °ÅÄ¥ ÇÊ¿ä ¾øÀÌ À̸¦ ¼¼Æ÷Ç¥¸é¿¡ ¹ßÇöÇÏ´Â ¹Ì»ý¹°À» »ý±Õ »óÅ·ΠÁ÷Á¢ »ýü ³»¿¡ Åõ¿©ÇÏ¿© Ç×±ÕÈ°¼ºÀ» ³ªÅ¸³»µµ·Ï ÇÔÀ¸·Î½á, Ç×±Õ ÆéŸÀ̵åÀÇ »ý»êºñ¿ëÀ» ÇöÀúÈ÷ ³·Ãß¾î Ç×±Õ ÆéŸÀ̵åÀÇ º¸±ÞÈ­¸¦ À¯µµÇÒ ¼ö Àִ  È¿°ú°¡ ÀÖ´Ù. ƯÈ÷ Àμö°øÅë Ç×»ýÁ¦ÀÇ °¡Ãà»ç¿ë Àü¸é ±ÝÁö¿¡ µû¶ó ³»¼ºÀÌ »ý±âÁö ¾Ê´Â »õ·Î¿î Ç×»ýÁ¦ ´ëüÀç ¹× ¸é¿ª °­È­¹°Áú·Î Ãà»ê¾÷¿¡ ÀÌ¿ëµÉ ¼ö ÀÖÀ» »Ó ¾Æ´Ï¶ó À¯¿ë¹°ÁúÀ» °æ±¸Åõ¿© ÇÒ ¼ö ÀÖ´Â ¹Ì»ý¹° Àü´Þü¸¦ °³¹ßÇÔÀ¸·Î½á ÇâÈÄ ÀǾàÇ° ¹× ´Ù±â´É¼º ¹Ì»ý¹° ±¸Ãà¿¡ Åä´ë°¡ µÉ °ÍÀ¸·Î ±â´ëÇÑ´Ù.


Âü°í¹®Çå

1. Conly J (2002) Antimicrobial resistance in Canada. CMAJ 167: 885–891.
2. Barton MD (2000) Antibiotic use in animal feed and its impact on human healt. Nutr Res Rev 13: 279–299.
3. Neu HC (1992) The crisis in antibiotic resistance. Science 257:1064–1073.
4. Makovitzki A, Avrahami D, Shai Y (2006) Ultrashort antibacterial and antifungal lipopeptides. Proc Natl Acad Sci U S A 103: 15997–16002
5. Lloyd DH (2012) Alternatives to conventional antimicrobial drugs: a review of future prospects. Vet Dermatol 23: 299–304
6. Mookherjee N, Hancock RE (2007) Cationic host defence peptides: innate immune regulatory peptides as a novel approach for treating infections. Cell Mol Life Sci 64: 922–933.
7. Jang SA, Kim H, Lee JY, Shin JR, Cho JH, et al. (2012) Mechanism of action and specificity of antimicrobial peptides designed based on buforin IIb. Peptides 34: 283–289.
8. Francisco JA, Earhart CF, Georgiou G (1992) Transport and anchoring of    betalactamase to the external surface of Escherichia coli. Proc Natl Acad Sci U S A 89: 2713–2717.




Total:45 page:(3/2)
29 Á¤º¸ ISBC »ý¹° ģȭÀûÀÎ Fe2O3 ¿äÅ©½© ÀÔÀÚÀÇ ´ë±Ô¸ð ¿¡¾î.. 18.09.04 2712
28 Á¤º¸ ISBC Ç×»ýÁ¦ »ý»ê ÃÖÀûÈ­¸¦ À§ÇÑ ¹æ¼±±Õ À¯ÀüÀû µµ±¸ .. 18.09.04 2230
27 Á¤º¸ ISBC Àúºñ¿ë °íÈ¿À² ¸ÂÃãÇü ¹Ì»ý¹° °Ë»ö Ç÷§Æû °³¹ß 18.09.04 5080
26 Á¤º¸ ISBC ÄÚ¸®³×¹ÚÅ׸®¿ò ±Û·çŸ¹ÌÄñ¿¡¼­ Çì¹Ì¼¿·ê·Î¿À½º .. 18.09.04 3276
25 Á¤º¸ ISBC Ç×»ýÁ¦ »ýÇÕ¼º ¹æ¼±±Õ À¯ÀüÀÚ ¹ßÇö Á¶Àý ±Ô¸í 18.09.04 2458
24 Á¤º¸ ISBC NK cell¿¡¼­ NF-¥êBÀÇ ´Ü°èÀû ÀλêÈ­¿Í Á¶ÀýÀ» Åë.. 18.09.04 3052
23 Á¤º¸ ISBC Cross linker¸¦ ÅëÇØ ¾ÈÁ¤È­µÇ´Â À¶ÇÕ ¾ËÆÄÇ︯½º.. 18.09.04 3558
22 Á¤º¸ ISBC Àü»çü ºÐ¼®°ú DNA-free CRISPR ½Ã½ºÅÛÀ» ÀÌ¿ëÇÑ .. 18.09.04 3804
21 Á¤º¸ ISBC Antisense RNA ±â¹Ý Á¤Á·¼öÀÎ½Ä Â÷´ÜÈ¿¼Ò °í¼Ó´Ù.. 18.08.30 3131
20 Á¤º¸ ISBC À¯ÀüÀÚ ¹ßÇö ÆÐÅÏÀ» ÀÌ¿ëÇÑ ¾à¹°Ç¥ÀûÀûÇÕ¼ºÀÇ °³.. 18.08.30 3035
19 Á¤º¸ ISBC ´ç °¨Áö °í°¨µµ FRET ´Ü¹éÁú ¼¾¼­ ¹× ¼ÒÇü °¨Áö .. 18.08.30 4063
18 Á¤º¸ ISBC ´ëÀå±Õ ÃÑÀ¯Àüü³»ÀÇ ArgR Àü»çÀÎÀÚ¿Í DNA °áÇÕü.. 18.08.30 2691
17 Á¤º¸ ISBC Á¾¾ç Ç÷°üÀÇ ½Å»ý°ú ÁøÇàÀ» È¿À²ÀûÀ¸·Î ¾ïÁ¦ÇÏ´Â .. 18.08.30 3278
16 Á¤º¸ ISBC Æ÷À¯¼¼Æ÷¿¡¼­ CRISPR Àü»ç ¾ïÁ¦ ÀåÄ¡¿Í À¯ÀüÀÚ È¸.. 18.08.30 2398
15 Á¤º¸ ISBC ¾ËÄÚ¿Ã ºÐÇØÈ¿¼Ò III (Alcohol Dehydrogenase III.. 18.08.30 2922
14 Á¤º¸ ISBC FACS ±â¹ÝÀÇ ÇÕ¼º Àΰ£ Ç×ü ÃÊ°í¼Ó ¹ß±¼ ½Ã½ºÅÛ .. 18.08.30 3714
[1] [2] [3]